Supporting Data for "Activity changes in neuron-astrocyte networks in culture under the effect of norepinephrine"

Monitoring the activity and morphology of Neuron-astrocyte networks in culture is powerful tool for studying dynamics, structure and communication in neuron-astrocyte networks independently or as a model of sub-brain network. These cultures are known to produce stereotypical patterns of activity e.g. highly synchronized network burst resembling sleep or seizure states, thus it enables to explore behaviors that can relate to brain function and disease. High-resolution microscopy of calcium imaging combined with simultaneous electrical recording provides a comprehensive overview on the networks dynamics. This set-up enables to apply global perturbations of electrical and chemical stimulation on the cultures during the recording task, and record the effects on network activity on line. Morphological changes in the cultures can be obtained to have a complete dataset for structure-function study of neuron-astrocyte networks in vitro.<br>The 4TB of data presented here was recorded and imaged as part of an accompanying study looking at in vitro structure-function of neuron-astrocyte networks. Simultaneous optical (calcium imaging) and electrical (MEA) recordings lasted 5-12 minutes and included spontaneous activity recording, electrical and chemical stimulation of neuron-astrocyte and isolated astrocyte cultures. Production procedures, experimental protocols and reuse options are included. The data has been suitable to reveal changes in the activity and morphology of the cultures and enabled observation and analysis of neuron-astrocyte and isolated astrocyte cultures behaviors under the applied perturbations.<br>Our dataset is sufficient to show significant changes in activity and morphology of neuron-astrocytes networks in culture under the applied stimulations. Over 100 recordings of 58 different cultures gives insight of the observations significance and led to conclusions about astrocyte activity and neuron-astrocyte network communication. Making it available here will allow others to test new tools for calcium imaging analysis and extracellular neuronal voltage recordings.