File S1 - Real Time RT-PCR Assays for Detection and Typing of African Horse Sickness Virus

Figures S1–S2 and Tables S1–S2. Table S1: Analytical sensitivity and efficiency of virus-species-specific (Seg-1 and Seg-3) and type-specific (Seg-2) assays with serially diluted infectious virus. Table S2: Analytical sensitivity and efficiency of virus-species-specific (Seg-1 and Seg-3) assays, with serially diluted dsRNA standards (RSArrah/01). Figure S1: Nucleotide alignments for the design of AHSV virus-species (Seg-1 and Seg-3) and type-specific (Seg-2) assays. Seg-1 and Seg-3 indicate the virus-species-specific assays while SRT-1 to SRT-9 indicate respective type-specific assays. The nucleotide sequence of primers and probes for individual assays, including redundant bases, are listed in Table 2. Figure S2: Slopes values in relation to the slope of the common regression line for virus-species and type-specific RT-PCR assays. Comparison of slopes of the nine reference strains to the respective common regression line in virus-species-specific (Seg-1 and Seg-3) and type-specific (Seg-2) assays with corresponding 95% CI. Slopes of all nine serotypes of AHSV are highly similar in each of the virus-species-specific or individual type-specific assays respectively. Solid red line represents common regression line while dotted lines mark defines the 95% CI.
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