Slc25a21_expression_profile. Slc25a21_expression_profile

omozygosity for Slc25a21 results in mice exhibiting orofacial abnormalities, alterations in carpal and rugae structures, and hearing impairment and inflammation in the middle ear. However, further analysis revealed that ablation of Slc25a21 was not causing these phenotypes and in fact the phenotypes appear to be caused by off target effects of components the targeting construct. We have shown with RT-PCR that one of the neighbouring genes, Pax9, is down regulated in mice homozygous for Slc25a21 . To increase the impact of the study and ensure the manuscript is more widely cited, we would like to explore other genes in the vicinity to see if their expression levels are also affected. RNAseq is the perfect vehicle for this analysis. We have RNA colelcted from at least 3 homozygous embryos for each of the alleles in the allelic series (tm1a, tm1b, tm1c adn tm1d). We will also process 8 wildtype samples (two from each allele type). All these samples have been collected at E13.5 as Pax9 is known to be expressed at this time point. We want to compare the expression levels of genes for each of these alleles to that of WT expression levels to further dissect the consequences of the tm1a allele. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/