Body weight, glucose and insulin levels in 4, 14 and 26 weeks db/db and Control mice as well as in 26 weeks C57Bl6 mice after 18 weeks fed with HFD.

Metabolic parameters including body weight, glucose and insulin levels were determined at the end of the experiments. Differences were detected by one-way ANOVA followed by Tuckey b or Tamhane tests as required. When we compared all groups under study we observed a significant increase in body weight as age progressed, and even higher body weights were observed in overweight mice (db/db 14 weeks old mice, db/db 26 weeks old mice and HFD treated mice), when compared to the rest of the groups [F(7,35) = 63.03, **p<0.01 vs. Control 14 weeks, Control 26 weeks, RD and 4 weeks old mice (db/db and Control), ††<0.01 vs. Control and db/db 4 weeks]. Glucose levels were significantly higher in db/db mice both at 14 and 26 weeks of age when compared with the rest of the groups [F(7,35) = 74.36, **p<0.01 vs. Control 14 weeks, Control 26 weeks, RD, HFD and 4 weeks old mice (db/db and Control)]. Increased insulin levels were observed in db/db 4 weeks mice, although these differences did not reach statistical significance. Significant hyperinsulinemia was observed in db/db 14 weeks and db/db 26 weeks whereas statistically higher levels were observed in HFD treated mice [F(7,35) = 11.498, **p<0.01 vs. Control 14 weeks, Control 26 weeks, RD and 4 weeks old mice (db/db and Control), ††p<0.01 vs. rest of the groups].