Investigation of gene mutations in canine alimentary large cell lymphoma. Canis lupus familiaris

Canine alimentary lymphomas are classified into large cell and small cell lymphomas. Both lymphomas are known to be of T-cell origin in most cases, but the molecular biological backgrounds have not been clarified. In human intestinal T-cell lymphoma, the mutations in genes related to JAK-STAT pathway have been frequently observed. In this study, gene mutations in canine alimentary large cell lymphoma were comprehensively investigated, especially focusing on those in genes associated with the JAK-STAT pathway. irst, whole exome sequencing (WES) analysis was performed using tumor samples from 4 dogs with alimentary large cell lymphoma. In addition to the gene mutations identified by WES, two hotspots of mutations in STAT3 and one in STAT5B were analyzed by targeted next-generation sequencing in 31 additional cases with alimentary large cell lymphoma. Finally, Sanger sequencing analysis was performed to investigate the mutations in the whole protein-cording region of SOCS3, a gene in which a mutation was identified by WES.