HeLa "Kyoto" cells under the scope

Name: HeLa “Kyoto” cells under the scope

Microscope: Perkin Elmer Operetta microscope with a 20x N.A. 0.8 objective and an Andor Zyla 5.5 camera.

Microscopy data type: The time-lapse datasets were acquired every 15 minutes, for 60 hours. From the individual plan images (channels, time-points, field of view exported by the PerkinElmer software Harmony) multi-dimension images were generated using the Operetta_Importer-0.1.21  with a downscaling of 4. 

Channel 1 : Low Contrast DPC (Digital Phase Contrast)

Channel 2 : High Contrast DPC

Channel 3 : Brightfield

Channel 4 : EGFP-α-tubulin

Channel 5 : mCherry-H2B

File format: .tif (16-bit)

Image size: 540x540 (Pixel size: 0.299 nm), 5c, 1z , 240t

Cell type: HeLa “Kyoto” cells, expressing EGFP-α-tubulin and mCherry-H2B ( Schmitz et al, 2010 )

Protocol: Cells were resuspended in Imaging media and were seeded in a microscopy grade 96 wells plate ( CellCarrier Ultra 96, Perkin Elmer). The day after seeding, and for 60 hours, images were acquired in 3 wells, in 25 different fields of view, every 15 minutes.

Imaging media: DMEM red-phenol-free media (FluoroBrite™ DMEM, Gibco) complemented with Fetal Calf Serum and Glutamax.

NOTE: This dataset was used to automatically generate label images in the following Zenodo entry:  https://doi.org/10.5281/zenodo.6140064

NOTE: This dataset was used to train the cellpose models in the following Zenodo entry: https://doi.org/10.5281/zenodo.6140111