Investigating human T cell development in the human pediatric thymus using scRNA-seq

To study human T cell development at the single cell level we profiled thymocytes from 3 pediatric donors using scRNA-seq. The data was subsequently used in the construction of a cell atlas of human thymic development across the life span. This allowed the identification of over 50 cell states in the human thymus and helped map the developmental trajectories of conventional and unconventional T cells throughout development. Overall design: Single cell transcriptional libraries were generated from human CD3+ and CD3- thymocytes obtained from pediatric donors using the 10X Genomics Chromium 3' sequencing V2 kit.