The effective concentrations giving 50% response (EC50) for the chrysenes tested in the yeast AhR signaling assay

In this experiment, an engineered Saccharomyces cerevisiae (YCM3) strain was used to detect ligand induced AhR signaling in order to compare the potencies of six mono-methylchrysenes to chrysene. Data are summarized (N = 3 experiments) and expressed as effective concentrations giving 50% response (EC50). Benzo[a]pyrene was added as a second reference PAH compound for purposes of comparison. Later, the AhR signaling response of chrysene was set to a Relative Potency Factor (RPF) of 1. Compounds with a potency greater than 1 were more effective as AhR ligands, and those <1 were weaker. The statistical significance of mean differences between chrysene and other compounds were determined by ANOVA test. These data were collected by December 2017 at Dr. Miller's Laboratory located at 1440 Canal Street, Suite 2110, New Orleans, LA 70112. This dataset supports the publication: Alqassim, A. Y., Wilson, M. J., Wickliffe, J. K., Pangeni, D., Overton, E. B., & Miller, C. A. (2019). Aryl hydrocarbon receptor signaling, toxicity, and gene expression responses to mono‐methylchrysenes. Environmental Toxicology, 34(9), 992–1000. doi:10.1002/tox.22770