mRNA-seq of DND-41 T-cell acute lymphoblastic leukemia cell line upon inhibition of hsa-miR-363-3p

The aim of this study was to investigate the effect of hsa-miR-363-3p on global gene expression in T-cell acute lymphoblastic leukemia (T-ALL). For this purpose we selected DND-41 T-ALL cell line characterized by high expression of this miRNA. The cells were transduced either with miRZip pGreenPuro scrambled vector (System Biosciences) or miRZip anti-hsa-miR-363-3p vector, each variant in three biological replicates. All 6 libraries were sequenced on Illumina NovaSeq6000 platform, in 2x150 mode, with a coverage of 60M paired reads/sample.