3'Pool-seq: an optimized cost-efficient and scalable method of whole-transcriptome gene expression profiling (Glitazone)

The advent of Next Generation Sequencing has allowed transcriptomes to be profiled with unprecedented accuracy, but the steep costs associated with full-length library sequencing have posed a limit on the accessibility and scalability of the technology. To address these limitations, we developed 3'Pool-seq, a simple, cost-effective, and scalable RNA-seq method that focuses sequencing to the 3' end of mRNA transcripts. Overall design: Transcriptomes (4 replicates each) from HepG2 Cells treated with Troglitazone, Pioglitazone, or Vehicle (DMSO) for 2 or 16 hours post-plating were compared to those that were left Untreated, and Differential Genes were determined using 3'Pool-seq.