Field diagnosis of bovine tuberculosis in the Sumapaz region. Phase 2: evaluation and validation of a rapid method based on isothermal amplification

This project is framed in the implementation of rapid and efficient bovine tuberculosis (TBB) diagnostic systems, with the objective of improving the productivity and sustainability of livestock production units in the Sumapaz region, ensuring the safety of dairy products intended for marketing both internally and in international markets. Traditionally, the diagnosis of TBB is made by applying Tuberculin, an indirect assay that fails to adequately predict active cases and shows cross-reaction with environmental mycobacteria. On the other hand, conventional laboratory diagnosis by microscopy of TBB, caused by Mycobacterium bovis, involves the isolation of acid-fast bacilli and Ziehl-Neelsen staining, which implies low sensitivity and long waiting periods between 6-8 weeks.