Gene expression and T cell repertoire profile at single cell level of peripheral blood T cells after treatment with a personalized neoantigen vaccine (GNOS-PV02) and Pembrolizumab for advanced hepatocellular carcinoma.

To further characterize the T cell response to the neoantigen vaccine, we performed single-cell RNA and T cell receptor (scRNA/TCR-seq) of peripheral blood T cells at the 12-week post-vaccination time point. T cells were isolated and enriched from the week 12 post-treatment PBMC of 3 patients (#6, 7, and 8) using the Miltenyi human Pan T Cell Isolation Kit and analyzed using scRNA/TCR-seq. ***Submitter declares that the raw data will be deposited in dbGap due to due to IRB restrictions***