LC-MS/MS data of C2C12 cells treated with Naringenin

To further clarify the reason for the upregulation of ERRγ expression by Naringenin(NAR), we biotinylated NAR for labeling. So, we used it to screen for proteins that directly interact with NAR. Lysates of C2C12 cells were co-incubated with biotin or NAR-biotin. Next, affinity cleavage magnetic beads were used to enrich the nonspecific proteins bound to biotin as well as specific proteins bound to NAR-biotin. The two sets of proteins obtained were separated by SDS-PAGE and analyzed by mass spectrometry (MS).