Quantitative Analysis of Wild Type,Ctnnb1 deltaEx3 and Ctnnb1 deltaEx2-6 Spinal Cord Transcriptomes

The goals of this study are to analyze NGS-derived spinal cord transcriptome profiling (RNA-seq) of wild type (WT), Nestin-Cre mediated Ctnnb1 Ex3 deletion (gain of function, dE3) and Ex2-6 deletion (loss of function, cKO) at embryonic day 13.5 (E13.5). Spinal cord RNA profiles were generated by deep sequencing, using Illumina Hiseq. The sequence reads that passed quality filters were analyzed at the transcript isoform level with HISAT2 and RSeQC. Spinal cord RNA profiles of E13.5 wild type (WT), Ctnnb1-dE3 and Ctnnb1-cKO mice were generated by deep sequencing, using Illumina Hiseq 2500.本研究旨在分析胚胎第13.5天（E13.5）的野生型（WT）、Nestin-Cre介导的Ctnnb1 Ex3缺失（功能获得型，dE3）以及Ex2-6缺失（功能丧失型，cKO）小鼠的下一代测序（Next-Generation Sequencing, NGS）来源脊髓转录组谱（RNA-seq）。本研究采用Illumina HiSeq平台开展深度测序以获取脊髓转录组数据；对通过质量过滤的序列读段，使用HISAT2与RSeQC在转录本异构体水平进行分析。此外，本研究同样通过Illumina HiSeq 2500平台完成深度测序，获取了E13.5野生型（WT）、Ctnnb1-dE3及Ctnnb1-cKO小鼠的脊髓转录组谱。