Cyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNA

Thhe dataset contains small interfering DNAs (siDNAs) that associated with MaAgo during heterologous expression in E. coli in absence or presence of MaACE1. The goal of the study was to 1) determine what type of siDNAs associate with MaAgo 2) from what sequences these siDNAs are derived and 3) whether MaACE1 influences the length or type of the siDNAs associated with MaAgo. Overall design: His-MBP-tagged-MaAgo was heterologously expressed in E. coli in presence or absence of MaACE1. His-MBP-tagged-MaAgo was purified using niNTA purification and subsequent Amylose purifcation. Nucleic acids were extracted from MaAgo, and a small DNA library was constructed as previoiusly described by Olovnikov et al. (https://pubmed.ncbi.nlm.nih.gov/24034694/). Detailed method are given in the associated publication.