RNA Sequencing of Meloidogyne incognita exposed to Nematicides

In this study, Meloidogyne incognita second stage juveniles were exposed to 4 different nematicides (oxamyl, fluopyram, fluazaindolizine, fluensulfone) in 4 replicates along with a water control for 24-hrs. After 24-hrs, nematodes were flash frozen in liquid nitrogen, total RNA was extracted, and Illumina 100bp single-end read libraries were made, and sequenced on Illumina HiSeq 3000 platform.

在本项研究中，对第二阶段米索迪尼科吉纳（Meloidogyne incognita）幼虫进行了暴露实验，分别接触了四种不同的杀线虫剂（甲酰胺、氟吡咯、氟嗪吡啶、氟苯磺酮），并设置水作为对照组，持续暴露时间为24小时。在24小时后，将线虫置于液氮中快速冷冻，提取总RNA，随后构建Illumina 100bp单端测序文库，并在Illumina HiSeq 3000平台上进行测序。