Figure_2.jpg

For the in vitro experiment four photoactivated increments were immersed in a cell culture medium with three different volumes and dilutions: 5.5 mL (Dilution 1 – D1), 11.0 mL (Dilution 2 – D2), or 22.0 mL (Dilution 3 – D3). A group of cells grown under ideal conditions treated exclusively with the basal medium was used as a control. A – Viability assay using Human keratinocytes (HaCaT) plated in quadruplicate for the MTT assay (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl bromide) tetrazolium]} for 24, 48, and 120 hours (h). B - Cell scratch migration test with HaCaT cells plated and evaluated at 0, 24, and 48 hours (h). C – Representative image of the cell migration analysis. Kruskal–Wallis test nonparametric test followed by Dunn's multiple tests. p < 0.05 was considered statistically significant. A:* means different from 24 hours group within the same treatment. B:* means different from 0-hour group within the same treatment.