Endogenous IFITM2 Involvement in Endocytosis to Regulate Neurogenesis

Purpose: To gain futher insight into how IFITM2 regulates the neurogenesis ,RNA-seq was used to analyze the genome-wide changes resulting from the cerebral cortices of E13 IFITM2 conditional knock out mice and littermate wild-type. Methods: Total RNA from E13 telencephalic tissue of wild-type(WT) and Ifitm2fl/fl;Nestin-Cre mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, total RNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 6000 platform in Annoroad Genomics Approximately one thousand transcripts showed differential expression between the wild-type(WT) and Ifitm2fl/fl;Nestin-Cre mice brain cortex, with a fold change =1.5 and p value <0.05. These results reflected IFITM2 plays a role in cortex development. Conclusions: RNA-seq based transcriptome characterization would provide a overall understanding how IFITM2 gene regulated the neurogenesis. Overall design: Total mRNA profiles of E13 wild type (WT) and Ifitm2 fl/fl;Nestin-Cre mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 6000.