Transcriptomic sequencing of brain regions in rat model exposed to spared nerve injury

In an effort to understand the underlying mechanisms concerning chronic pain, a rodent model was utilized to identify molecular signals and indications of chronic pain over time after spared nerve injury. The insular cortex, cingulate cortex, thalamus, amygdala, and somatosensory cortex was collected at 1, 3, 7, 14, and 21 days post-injury and from sham animals. The RNA was extracted and underwent mRNA sequencing,