Nick Ferenz, Patricia Wadsworth (2012) CIL:40266, Sus scrofa domestica, kidney epithelial cell. CIL. Dataset
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Time lapse movie of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at metaphase in a vertical strip between the metaphase plate and spindle pole. The activated strip moves poleward, demonstrating a marked microtubule flux during mitotic metaphase.
See Fig 1A in: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002
时间流逝电影展示了稳定表达光激活型GFP-微管蛋白的猪肾LLCPK1细胞的图像。荧光在减数中期沿垂直带被激活，该带位于中期板与纺锤体极之间。激活的带向纺锤体极移动，展示了明显的微管流量变化，这体现了有丝分裂中期的微管动态变化。详见NP Ferenz和P Wadsworth于2007年发表的《哺乳细胞中前期微管阵列表现出类似流量的行为》一文中图1A。Mol Biol Cell 18:3993-4002
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