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Sequencing analysis of m6A-methylated RNAs responding to UV

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE92867
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m6A RNA immunoprecipitation experiments were conducted to identify RNAs that are directly m6A-methylated by the METTL3 methylatransferase specifically in response to UV. WT and METTL3 KO U2OS cells were subjected (plus UV condition) or not (minus UV condition) to a 50 J dose of UVC irradiation. Following irradiation, cells were lyzed and total RNAs were extracted. Poly A+ RNAs were isolated and subjected to m6A RNA precipitation.
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2019-05-15
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