ChIP-seq and RNA-seq analysis of histone Kcr in the embryonic forebrain and neural stem/progenitor cells.

To gain a deeper insight into how histone lysine crotonylation regulates embryonic neural stem/progenitor cells (NSPCs) proliferation and differentiation, ChIP-seq and RNA-seq were performed to analyze the genome-wide changes of histone Kcr and transcriptiome changes stimulated with crotonate and MS-275 in E13.5 NSPCs. Total RNA was extracted from E13.5 NSPCs before and after crotonate and MS-275 stimulation and generated by deep sequencing, in four biological replicates, using Illumina novaseq 6000. ChIP was excuted using pan-Kcr and Pan-Kac antibody with E13.5 NSPCs before and after crotonate and MS-275 stimulation.