Cell Cycle analysis of HeLa cells exposed to colibactin with inhibitors

Cells exposed to the genotoxin colibactin, which is produced by certain strains of E. coli and other proteobacteria, typically exhibit cell cycle arrest as a result of colibactin-induced DNA damage. In this experiment, cells were exposed to either colibactin-producing E. coli, non-colibactin producing E.coli, or colibactin-producing E.coli treated with a putative colibactin-biosynthesis inhibitor. If the inhibitor is effective, those cells should not exhibit the same level of cell cycle arrest as cells which were exposed to colibactin-producing E. coli without an inhibitor.
Conclusion:
Cells exhibited a decrease in cell-cycle analysis in a dose-dependent fashion when treated with the inhibitor, as expected. Thus we conclude that the inhibitor is interfering with colibactin biosynthesis.