Isolated TEC and DC from human iPSc-derived thymic organoids (hTOs) seeded with primary early thymic progenitors (ETPs) at late phase of maturation (D28)

We employed an ultra-sensitive bulk RNA sequencing approach to study thymic epithelial cells (TECs), sorted by FACS based on their HLA-DR expression levels, and dendritic cells (DCs), both isolated from hTOs at the late stage of differentiation (day 28). These hTOs were generated by reaggregating iPSc-derived thymic epithelial progenitors (TEPs) with primary ETPs. Overall design: At D28 of organoid formation, cells were isolated to obtain the CD45-EPCAM+ HLA-DRhigh and HLA-DRlow fractions (TEChi and TEClo), as well as the CD45+EPCAMlo fraction (DC), analyzed using SMARTER bulk RNA-seq