RNA-seq in fetal mice brain following c-Flip knockdown

We characterized the comprehensive set of differentially expressed genes resulting from the knockdown of c-Flip in the E13.5 fetal head and identified the transcriptional profile of ZIKV-induced DEGs in the context of c-Flip deficiency Female wild type mice aged 8 to 10 weeks and male c-Flip+/- mice aged 10 to 12 weeks were set up for timed-matings and at embryonic days (E5.5) pregnant mice were inoculated with 2 mg of anti-IFNAR antibody (MAR1-5A3, Leinco Technologies). At E6.5, mice were infected intraperitoneally (i.p.) with 200ul of 1*10^6 plaque forming unit (PFU), and the mock-infected mice were injected with 200ul PBS as control. Mice in each group were observed daily for the signs of disease and death for 7 days post infection (dpi). They were sacrificed at E13.5 (dpi), and the fetal brains were collected for viral load study and transcriptome analysis.