Gene expression in wild-type and transgenic plants overexpressing rice OsTOP6A1 gene

10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing rice topoisomerase 6 subunit A1; OsTOP6A1) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Keywords: Arabidopsis, wild-type and transgenic seedlings, differential gene expression The experiment involves the study of differential gene expression in transgenic Arabidopsis seedlings overexpressing rice topoisomerase 6 subunit A1 (OsTOP6A1) cDNA as compared to wild-type.Three biological replicates for each sample were used for microarray analysis.