ScRNA-seq of adult rat testes with different androgen enviroment. ScRNA-seq of adult rat germ cells

Spermatogenesis is an efficient, complex, and highly organized proliferation and differentiation process, which relies on multiple factors, including testosterone produced by Leydig cells. Although the critical role played by testosterone in spermatogenesis is well recognized, the mechanism by which it works is still not completely understood, partially due to the inability to specifically and precisely monitor testosterone-dependent changes within developing germ cells. Here we present 4 samples of single-cell RNA sequencing data (10,983 adult rat testicular cells) that covered a complete testosterone depletion and restoration circle. The dataset includes all developing germ cells from spermatogonia to spermatozoa and somatic Sertoli and Leydig cells and is ideal for characterizing all developmental germ cells, their regulatory networks, and novel stage-specific markers. The dataset is particularly useful in exploring the effects of the androgen environment on regulation of spermatogenesis. As this is the first single-cell RNA-Seq dataset for rat testes, it can also serve as a reference for future studies involving.