Expression data from rice roots treated with N starvation for 1 h

We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by N starvation (1 h) in rice roots. Transcriptomic analysis of rice roots revealed that the expression of 288 genes was differentially regulated (144 up, 144 down) by N starvation (1 h). Total RNA extracted from roots of 10-day-old rice seedlings grown in Yoshida's hydroponic solution (+N) and treated with N starvation for 1 h (-N) was used for microarray analysis. RNA samples from three biological repeats were sent to the Affymetrix Gene Expression Service Lab at Academia Sinica, Taipei, Taiwan (http://ipmb.sinica.edu.tw/affy/) for target preparation, and hybridization to the GeneChip Rice Genome Array.