RNA sequencing. Homo sapiens

To understand the mechanism by which corylin ameliorates cellular senescence in HUVECs, we next used RNA sequencing to analyze transcriptome differences in three different groups: proliferating cells (Y), senescent cells (S), and senescent cells with corylin treatment (S+C). First, we compared the S:Y (S/Y), S+C:S (S+C/S), and S+C:Y (S+C/Y) groups to verify the transcriptome changes between them. Next, we focused on 433 shared transcripts between S/Y and S+C/S . By comparing the KEGG pathway annotation, we found shared pathways between the two groups associated with cellular senescence, the cell cycle, DNA replication, and the p53 signaling pathway . We next decoded the transcriptome differences in those pathways. Numerous transcripts were shifted in S/Y, such as a reduction in cyclin protein, replication factor, DNA helicase, cell proliferation signals, and an increase in SASP, indicating that HUVECs are in the senescent state