DataSheet_1_Responses of differential metabolites and pathways to high temperature in cucumber anther.docx

Cucumber is one of the most important vegetable crops, which is widely planted all over the world. Cucumber always suffers from high-temperature stress in South China in summer. In this study, liquid chromatography–mass spectrometry (LC-MS) analysis was used to study the differential metabolites of cucumber anther between high-temperature (HT) stress and normal condition (CK). After HT, the pollen fertility was significantly reduced, and abnormal anther structures were observed by the paraffin section. In addition, the metabolomics analysis results showed that a total of 125 differential metabolites were identified after HT, consisting of 99 significantly upregulated and 26 significantly downregulated metabolites. Among these differential metabolites, a total of 26 related metabolic pathways were found, and four pathways showed significant differences, namely, porphyrin and chlorophyll metabolism; plant hormone signal transduction; amino sugar and nucleotide sugar metabolism; and glycine, serine, and threonine metabolism. In addition, pollen fertility was decreased by altering the metabolites of plant hormone signal transduction and amino acid and sugar metabolism pathway under HT. These results provide a comprehensive understanding of the metabolic changes in cucumber anther under HT.

黄瓜作为全球范围内广泛种植的重要蔬菜作物之一，其重要性不言而喻。在我国南方地区，黄瓜常在夏季遭受高温胁迫。本研究采用液相色谱-质谱联用技术（LC-MS）对高温胁迫（HT）与正常条件（CK）下黄瓜雄蕊的差异代谢物进行了研究。高温处理后，花粉的育性显著降低，通过石蜡切片观察发现雄蕊结构异常。此外，代谢组学分析结果表明，在高温处理后，共鉴定出125种差异代谢物，其中99种代谢物显著上调，26种代谢物显著下调。在这些差异代谢物中，共发现26条相关代谢途径，其中四个途径表现出显著差异，分别是叶绿素和卟啉代谢；植物激素信号转导；氨基糖和核苷酸糖代谢；以及甘氨酸、丝氨酸和苏氨酸代谢。此外，在高温胁迫下，通过改变植物激素信号转导和氨基酸及糖代谢途径中的代谢物，花粉育性降低。这些结果为高温胁迫下黄瓜雄蕊代谢变化提供了全面的认识。