File S1 - PIAS1 Regulates Breast Tumorigenesis through Selective Epigenetic Gene Silencing

Contains the following files: Figure S1, Table S1 and Table S2. Figure S1. Validation of the polyclonal anti-PIAS1 antibody by immunofluorescence. MDA-MB231 cells containing control shRNA or PIAS1 shRNA2 were fixed by 3 different methods as indicated, followed by staining with polyclonal anti-PIAS1. FMA, formaldehyde. Table S1. Microarray analysis. Fold induction is defined as the ratio of the expression levels of a given gene in PIAS1 shRNA2 vs. control shRNA cells. Genes with greater than 10-fold induction under Stem Cell Media (SCM) condition are shown. Table S2. Primers used for Q-PCR, ChIP and methylation.
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