Transcriptomic Analysis of Sorghum bicolor L. Hwanggeumchal Under Various Abiotic Stress Conditions

This project investigates the transcriptomic responses of Sorghum bicolor L. Hwanggeumchal to various abiotic stresses, including salinity (150 mM NaCl), drought (20% polyethylene glycol 8000), heat (40 degrees Celsius), cold (4 degrees Celsius), and abscisic acid (100 uM ABA). Seeds were surface-sterilized and grown in soil under controlled chamber conditions (16-hour photoperiod, 25/23 degrees Celsius day/night temperature). Two-week-old plants were subjected to stress treatments, and leaf samples were collected at 6, 12, and 24 hours after exposure. Non-treated control samples were collected at corresponding time points. The RNA sequencing libraries were prepared and sequenced using paired-end (2 x100 bp) Illumina NovaSeq 6000 technology. This study aims to uncover the molecular mechanisms underlying abiotic stress tolerance in sorghum by analyzing differentially expressed genes and related pathways.