Dataset to A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3

The main challenge for the success of directed enzyme evolution campaigns is the availability of suitable high-throughput screening systems. Multiplex capillary electrophoresis with a universal UV-based product detection is a promising analytical tool, which allows to quantify product formation simultaneously in 96-capillaries. A 96-multiplexed capillary electrophoresis (MP-CE) enables a throughput that is comparable to traditional direct evolution campaigns employing 96-well microtiter plates. The platform allows a comprehensive view on enzyme activity through the detection of all products formed during the target reaction. In the manuscript A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3. (Gärtner, A., Ruff, A.J. & Schwaneberg, U., Sci Rep 9, 15479 (2019), doi:10.1038/s41598-019-52077-w), we report for the first time the usage of a MP-CE system for the screening of evolution libraries. After adaptation of the screening preparation and measurement conditions, the system was validated for the suitability as screening platform for P450 BM3 evolution. The oxidation of α-isophorone was selected as target reaction. The MP-CE platform was compared to the standard cofactor (NADPH) screening system and was found to be 3.5-fold more efficient in identification of beneficial. Here the dataset to the above mentioned manuscript is reported. Source Data of the figures are given in Origin and Excel format to display the CE-measurements (CE-chromatograms), the screening data (summary variants and 1st screening round) and how the data evaluation described in the MS (standard curves and standard deviation) was performed. In the description of dataset a summary of the collected source data is given.