Methods for Culturing Adult CNS Neurons Reveal a CNS Conditioning Effect

To develop a successful, reliable and scalable method for establishing primary cultures of adult neurons from all regions of the brain. Using adult motor cortex cultures, we identify a CNS "conditioning" effect after spinal cord injury. In vivo conditioning was administered 24hrs prior to cultures. Comparative gene expression profiling analysis of RNA-seq data for primary cortical neurons that received an in-vivo conditioning lesion 24hrs prior to plating.