Characterization of RNAs in soybean (Glycine max)

RNA-seq was used to characterize gene expression in soybean from a wide range of tissues. The primary focus of the project was small RNAs, and the identification of microRNAs and phased siRNA-generating loci, but RNA-seq data were generated from the same samples. This project was supported by the United Soybean Board. Overall design: mRNA profiling was done by Illumina TruSeq sample preparation (not stranded) followed by high-throughput sequencing using an Illumina HiSeq 2500 at the Delaware Biotechnology Institute. The study focused on small RNA, with these RNA-seq libraries made from the same samples (see PMID: 25465409).