ldl3 mutant vs wild-type in Arabidopsis de novo shoot regeneration_RNA-seq and ChIP-seq

Genome-wide histone modifications and gene expression profiles of Arabidopsis root explants from ldl3 and wild-type (Col-0) seedlings were assessed during de novo shoot regeneration process. Root explants (0-1cm from the root tip) were excised from seedlings 6 days after germination, and cultured on auxin-rich callus-inducing medium (CIM) for 14 days and subsequently on cytokinin-rich shoot-inducing medium (SIM). The root explants were collected on days 0 and 14 on CIM, days 1 and 7 on SIM, and were subjected to RNA-seq and ChIP-seq.