Data and scripts from common garden and reciprocal transplant experiments investigating traits associated with altitude and temperature in Heliconius erato and Heliconius melpomene
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Two Excel files contain the data: CGE_all_data.xlsx: This excel file contains data collected on two species of Heliconius butterflies (Heliconius erato and Heliconius melpomene) reared under lab conditions (21 degrees celcius) - The Common Garden Experiemtn. Individuals were offspring of wild-caught females (Mother_Collection_Information) collected in across an elevational gradient (between 300 and 1500 masl). Data was collected on developmental parameters (CGE_development_data) including survival, developmental time and weight. Once emerged as adults individuals were tested for time to cold knockdown at 5 degrees celcius and recovery and time to heat knockdown at 39 degrees celcius and recovery (CGE_Thermal_tolerance). Wings were then removed and their size and colour parameters measured (RTE_Wing_data) RTE_Data_all.xlsx: This excel file contains data collected on two species of Heliconius butterflies (Heliconius erato and Heliconius melpomene) reared under two temperature conditions (19 and 25 degrees celcius) - The Reciprocal Trnasplant Experiment. Individuals were offspring of wild-caught females (Mother_Collection_Information) collected in 2 elevational ranges (300-600 masl as "low" and 1100-1500 masl as "high"). Data was collected on developmental parameters (RTE_development_data) including survival, developmental time and weight. Once emerged as adults individuals were either tested for time to cold knockdown at 5 degrees celcius and recovery (RTE_Cold_tests) or time to heat knockdown at 39 degrees celcius (RTE_Heat_tests). Wings were then removed and their colour parameters measured (RTE_Wing_data) Two associated R scripts that were used the analyse the data from the experiments: modelling_protocol.R: R script used to fit the Linear Mixed-Effect or Generalised Linear Mixed-Effect Models to the data and calculate effet sizes WingColour_image_processing.R; R script used to analyse the wing images to calculate the percentage of the wing that was black and the reflectance of the red patch.



