Comparative analysis of excretory–secretory products of muscle larvae of three isolates of Trichinella pseudospiralis by the iTRAQ method

we compared the differentially expressed proteins of excretory-secretory (ES) products in three isolates of T. pseudospiralis ML [muscle larvae from Russia (RUS), United States of America (USA) and Australia (AUS)] using isobaric tags for relative and absolute quantification (iTRAQ)-based technology.A total of 2,591 nonredundant proteins were identified, of which 65 (146), 72 (98) and 43 (103) significantly upregulated (downregulated) differentially expressed proteins were detected among pairwise comparisons (T4RUS vs T4USA, T4AUS vs T4USA and T4RUS vs T4AUS). In addition, GO annotation, KEGG and STRING analyses were carried out on the screened differentially altered proteins.