Tick pathogen status (DP1.10092.001)

A target of 130 individual ticks per site per year are selected for pathogen testing, with the total numbers tested in a given site-year dependent on availability. Ixodes scapularis and Ixodes pacificus (if available beyond what is used for archive) nymphs are targeted for testing of Anaplasma phagocytophilum, Babesia microti, Ehrlichia muris-like agent, Borrelia burgdorferi sensu lato, Borrelia miyamotoi, and Borrelia mayonii. Nymphs in the genera Amblyomma and Dermacentor (if available beyond what is used for archive) are targeted for testing of Francisella tularensis, Rickettsia rickettsia, Anaplasma phagocytophilum, Ehrlichia chaffeensis, Ehrlichia ewingii, and Borrelia lonestari. Instrumentation:本研究设定每采样点每年的目标采样量为130只蜱虫用于病原体检测，特定采样点年度的实际检测总数则取决于样本可获取情况。针对肩突硬蜱（Ixodes scapularis）以及太平洋硬蜱（Ixodes pacificus，若存有超出存档需求的若虫样本），需对其开展嗜吞噬细胞无形体（Anaplasma phagocytophilum）、微小巴贝斯虫（Babesia microti）、类鼠埃里希体（Ehrlichia muris-like agent）、伯氏疏螺旋体复合群（Borrelia burgdorferi sensu lato）、宫本疏螺旋体（Borrelia miyamotoi）以及马约恩疏螺旋体（Borrelia mayonii）的检测。对于花蜱属（Amblyomma）与革蜱属（Dermacentor）的若虫（若存有超出存档需求的样本），需对其进行土拉弗朗西斯菌（Francisella tularensis）、立氏立克次体（Rickettsia rickettsia）、嗜吞噬细胞无形体、查菲埃里希体（Ehrlichia chaffeensis）、尤因埃里希体（Ehrlichia ewingii）以及孤星疏螺旋体（Borrelia lonestari）的检测。检测仪器：