DSB-induced transcription of pLac-Tet plasmid in cell-free extracts.

Raw reads of RNA products generated upon incubation of circular and linear pLac-Tet plasmid in human cell-free extract. Overall design: RNA profiles of products generated upon incubation of circular and linear pLac-Tet plasmid in human cell-free extract obtained by deep sequencing using Ion Proton. pLac-Tet plasmid sequence prepared by cloning a fragment of 556 bp which contains an I-SceI site flanked by 3 Tet and 8 Lac repetitive elements into SfiI site of pMK-RQ vector (GENEART).