Low biomass microbiology samples collected from a hydraulically fractured well producing from the Niobrara Shale in Colorado

Low biomass waters provide a unique challenge in the field of microbial ecology. It is difficult to determine, when biomass concentrations are extremely low, whether or not the sequencing data received are of good quality and representative of the waters sampled. Fifty-nine samples including 8 blanks were collected from a low biomass hydraulically fractured well producing from the Niobrara Shale in Colorado. At least 4 samples were collected by filtering the exact volume for each listed here: 1000 mL, 900 mL, 800 mL, 700 mL, 600 mL, 500 mL, 400 mL, 300 mL, 200 mL, 100 mL, 0 mL (blanks).