Ikzf2/helios mutant cochlear RNA-seq analysis

Given the potential role of the helios transcription factor in postnatal outer hair cell gene regulation indicated by our RiboTag translatome analyses, our next goal was to identify and validate genes that are regulated by helios in outer hair cells by performing RNA-seq on P8 cello mutant animals compared to wild-type littermates. Difference in cochlear gene expression between wild-type Ikzf2+/+ animals and mutant Ikzf2cello/cello littermates was analyzed by RNA-seq in biological duplicates.基于我们的RiboTag翻译组分析（RiboTag translatome analyses）所揭示的Helios转录因子（helios transcription factor）在出生后外毛细胞基因调控中的潜在作用，本研究的下一目标为：对出生后8天（P8）的cello突变体动物与其野生型同窝幼崽开展RNA测序（RNA-seq），以鉴定并验证外毛细胞中受Helios调控的基因。随后，我们通过设置生物学重复的RNA-seq实验，对比分析了野生型Ikzf2+/+动物与突变型Ikzf2cello/cello同窝幼崽的耳蜗基因表达差异。