Transcriptome-wide m6A Methylation Profiling of dHL-60 human neutrophils by m6A-seq

By performing m6A-seq analysis on dHL-60 human neutrophils infected with Escherichia coli, we want to identify the m6A methylation pattern in neutrophils after bacteria challenge. m6A-seq analysis revealed that the consensus m6A motifs were most significantly enriched within the m6A peaks with typical m6A peak distribution features, and m6A methylation sites were located primarily in the protein coding sequence region and 3' untranslated region of mRNA transcripts, in neutrophils with bacterial infection. Overall design: Identifying the m6A methylation pattern in dHL-60 human neutrophils infected with Escherichia coli. Two biological replicates.