Gene expression analysis of WT and Keap1–/– MEFs in vitro culture condition (2D) and in allograft condition (3D). Mus musculus

WT TR MEFs and Keap1–/– TR MEFs were established according to the following method. Keap1+/– mice were mated, and WT and Keap1–/– embryos were collected at embryonic day 13.5. Primary MEFs were immortalized by expression of SV40 T antigen. The immortalized MEFs were transformed by expression of HRAS G12V to generate WT TR MEFs and Keap1–/– TR MEFs. Overall design: Total RNA from WT-TR MEFs and Keap1–/–-TR MEFs was prepared for in vitro culture conditions (2D) and allograft conditions (3D). To prepare the total RNA from the 3D conditions, 1 x 10^7 WT-TR MEFs and 3 x 10^6 Keap1–/–-TR MEFs were injected into nude mice, and the tumors were harvested for extraction of total RNA when their volumes reached 100-150 mm^3.