Supplementary material for manuscript submission at Ecology and Evolution

Supplementary data for the manuscript submission at Ecology and Evolution entitled: "<i>Gaps and data ambiguities in DNA reference libraries: A limiting factor for molecular-based biodiversity assessments using annelids as a case study</i>"<b>Checklists:</b>OBIS Arabian Checklist (CL-MTAP)OBIS Mediterranean Checklist (CL-MTMS)Wehe &amp; Fiege (2002) Polychaeta Checklist from the Arabian Peninsula (CL-MTAWF)<b>FASTA datasets</b>BOLD Annelida at species level-only COI dataset (DS-MTWA): https://dx.doi.org/10.5883/DS-MTWA ARMS Annelida Metabarcoding ASV dataset<b> (</b>DS-ARMS)MZGdb Annelida Indian Ocean (MZGDB)MZGdb Annelida Indian Ocean + ARMS Annelida Metabarcoding ASV dataset (MZGdb_+_DS-ARMS)ARMS Species level IDs-only + complexes in the database (ASV-complex)<b>Supplementary Tables and Figures:</b><b>Table S1</b><b>:</b> Obtained ASVs and respective number of reads per ARMS and taxonomic identification based on RDP method.<b>Table S2</b><b>:</b> Database COI accession numbers and respective references used in Fig. 5<b>Table S3: </b>Detailed list of the obtained Genera and Families from DS-ARMS. All belonging to Polychaeta, unless stated otherwise.<b>Fig. S1</b><b>:</b> Neighbour-Joining phylogenetic Tree using Kimura 2-parameters model for DS-ARMS. All 5375 ASVs are individually shown with the respective taxonomic assignment (RDP). Discordant clades (based on ASAP methodology) highlighted in red. The largest MOTU, identified as “Dorvilleidae”, is highlighted in green and match COI sequences of <i>Dorvillea cf. similis</i> from our custom library in development. A section corresponding to the “high taxonomic ambiguity” suspected of being pseudogenes is also displayed.Public DOIs for specific BOLD related datasets will be available upon paper acceptance.