Data underlying the publication: DNA Wrapping by a Tetrameric Bacterial Histone

The research objective was to study how histone HLp from Leptospira perolatii binds and structures DNA. We have studied this with light microscopy, electrophoretic mobility shift assays, micrococcal nuclease assays, DNA topology assays, ligase-mediated circularisation, tethered particle motion (TPM), X-ray crystallography, and molecular dynamics (MD) simulations. This dataset contains the raw data of obtained root mean squared displacement (TPM).

本研究旨在探究佩罗拉特钩端螺旋体（Leptospira perolatii）来源的组蛋白HLp与DNA的结合方式及DNA结构的构建机制。我们采用光学显微镜、电泳迁移率变动分析、微球菌核酸酶实验、DNA拓扑学分析、连接酶介导的环化实验、束缚粒子运动（tethered particle motion，TPM）、X射线晶体学以及分子动力学（molecular dynamics，MD）模拟等多种技术手段对该课题进行了研究。本数据集收录了束缚粒子运动（TPM）实验所获得的均方根位移（root mean squared displacement）原始数据。