Phylogenomics supports a single origin of terrestriality in Isopods.

Terrestriality, the adaptation to life on land, is one of the key evolutionary transitions, occurring numerous times across the tree of life. Within Arthropoda, there have been several independent transitions: in hexapods, myriapods, arachnids and isopods. Isopoda is a morphologically diverse order within Crustacea, with species adapted to almost every environment on Earth. The order is divided into 11 suborders with the most speciose, Oniscidea, including terrestrial isopods such as woodlice and sea-slaters. Recent molecular phylogenetic studies have challenged traditional isopod morphological taxonomy, suggesting that several well-accepted suborders, including Oniscidea, may be non-monophyletic. This implies that terrestriality may have evolved multiple times. Current molecular hypotheses, however, are based on limited sequence data. Here, I collate available genome and transcriptome datasets for 36 isopods and four peracarid crustaceans from public sources, generate assemblies, and u..., Data was collated from public sources, genomic and transcriptomic data were assembled, then analysed with phylogenetics and molecular-dating., , # Phylogenomics supports a single origin of terrestriality in Isopods.

[https://doi.org/10.5061/dryad.prr4xgxv8](https://doi.org/10.5061/dryad.prr4xgxv8)

Alignment files for each dataset analysed.

## Description of the data and file structure

Amino-acid alignment files for phylogenomic dataset aligned with mafft or fsa, with and without trimming:

`Isopoda.aa.fsa.phy`

`Isopoda.aa.fsa.trim.phy`

`Isopoda.aa.mafft.phy`

`Isopoda.aa.mafft.trim.phy`

Nucleotide alignment files for phylogenomic dataset aligned with mafft or fsa, with and without trimming:

`Isopoda.nt.fsa.phy`

`Isopoda.nt.fsa.trim.phy`

`Isopoda.nt.mafft.phy`

`Isopoda.nt.mafft.trim.phy`

Nuclear ribosomal alignments, indicating non-aligned, removed sections of genes for marker-gene dataset:

`Isopoda.ribosomal.uncut.nex`

Nucleotide alignment file including all genes, for marker-gene dataset: 

`Isopoda.marker-gene.phy`

Scripts for figures:

`Scripts.zip`

Available on Zenodo: [https://doi.org/10.5281/zenodo.1338352...