Lung proteome and metabolome endotype in HIV-associated obstructive lung disease

Purpose: Obstructive lung disease is increasingly common among persons with HIV in both smokers and non-smokers. We used aptamer proteomics to identify proteins and associated pathways in HIV-associated obstructive lung disease.
Methods: Bronchoalveolar lavage fluid (BALF) samples from 26 persons living with HIV with obstructive lung disease were matched to persons living with HIV without obstructive lung disease based on age, smoking status, and antiretroviral treatment. 6,414 proteins were measured using SomaScan aptamer-based assay. We used sparse distance-weighted discrimination (sDWD) to test for a difference in protein expression and permutation tests to identify univariate associations between proteins and forced expiratory volume in 1s precent predicted (FEV1pp). Significant proteins were entered into a pathway overrepresentation analysis (ORA). We also constructed protein-driven endotypes using K-means clustering and performed ORA on the proteins that were significantly differe..., ,