Transcriptome Analysis of Wild Type and OsMYB305 Overexpressed Rice

The goals of this study are to identify the differentially expressed genes in the roots of OsMYB305 overexpressed Rice growing under low nitrogen condition. Overall design: Root mRNA profiles of wild-type (WT) and OsMYB305 overexpressed Rice growing under low nitrogen condition for 14 days were generated by deep sequencing, in bipartite, using HiseqXten-PE150. The sequence reads were first filtered with the FastQC v0.11.8;then pre-processed using the Trimmomatic-0.38;then mapping with Hisat2 v2.1.0;finally differentially-expressed genes(DEGs) were identified using the Cufflinks v2.2. OE15 and OE20 are independent transgenic lines of OsMYB305 which have very similar phenotypes compared to WT under low N treatment: growth advantages, increased N concentrations, decreased cellulose contents, etc.