Gene expressions of IBMX-induced neuron differentiation from placenta-derived multipotent stem cells

Little is known about factors that induce stem cells differentiated into astrocyte. Using double cross strategy, we effective narrow down the candidate molecules crucial for astroglial differentiation. By cross comparison the data from transcriptomic and proteomic experiments, we selected 24 candidate genes which might be involved in the neuron differentiation. The 24 genes were further cross compared with Alzheimer disease (AD) database, which regarded as a neuron degenerative database. There were 18 out of 24 candidates showed opposite expression level in our data and AD database. Among them, the combination of Heat Shock Protein 27 (HSP27) and S100 calcium-binding protein A16 (S100A16) showed predominant crucial in neuronal differentiation than other gene combination. We previously showed that the expression level of HSP27 directly related to the glutamatergic neuron differentiation under IBMX induction. In this paper, we found co-silence of HSP27 and S100A16 directly induced PDMCs differentiate into functional glutamatergic neurons and astrocytes while IBMX is no longer required in this process. The resulting astrocyte not only exhibit the classic morphology but also with astrocyte function. We used our existing neuron differentiation model derived from placenta-multipotent stem cells (PDMCs) as a cell model. The neuron differentiation was induced by adding 0.4 mM IBMX. The differentiated neurons were harvested 12h and 24h after induction , and the mRNAs were collected by RNeasy Mini Kit (Qiagen, Hilden, Germany), and mRNAs were used for probing of a human oligonucleotide microarray (Agilent SurePrint G3 Human GE 8x60K oligonucleotide microarrays; Agilent Technologies, USA).